Mini-percoll gradient may be used for frozen-thawed sperm selection in sheep
نویسندگان
چکیده
منابع مشابه
Comparison of CapriPure and Percoll density gradients for sperm separation of frozen- thawed goat spermatozoa
Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...
متن کاملComparison of Different Sperm Selection Techniques in Ram Frozen - Thawed Sperm
Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evalu...
متن کاملComparison of Motility, Morphology, Acrosome Integrity, Membrane Integrity and Fertilizing Ability of Frozen-Thawed Buffalo Sperm Separated by a Percoll
The aim of this study was to evaluate sperm quality following separation and selection using a silane-coated silica colloid method (PureSperm ® ) or Percoll ® . Frozen/thawed sperm from buffalo bulls (n=5) were assigned to two groups. The first group was separated using a Percoll ® discontinuous gradient (45 and 90%) and the second group by a single layer of PureSperm ® . Sperm motility, concen...
متن کاملMotility and fertilizing ability of frozen-thawed raM sperM froM two sheep breeds
The aim of the study was to evaluate motility parameters of Lacaune (Lc) and east-Friesian (eF) ram frozen-thawed spermatozoa by cASA (computer-Automated Sperm Analysis) technique in relation to their in vivo fertilizing ability. Fresh ejaculates were diluted in a Triladyl extender containing 20 % egg yolk, 6 % glycerol and 0.1 % trehalose and frozen in a liquid nitrogen vapour. The evaluation ...
متن کاملFertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods
The aim of the project was to use current simple and practical laboratory tests and compare results with the foaling rates of mares inseminated with commercially produced frozen semen. In Exp. 1, semen was tested from 27 and in Exp. 2 from 23 stallions; 19 stallions participated in both experiments. The mean number of mares per stallion in both experiments was 37 (min. 7, max. 121). Sperm morph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
سال: 2019
ISSN: 1678-4162,0102-0935
DOI: 10.1590/1678-4162-9807